SKT-050

Human High-Sensitivity Cardiac Troponin T CLIA Kit

Description

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The Human High-sensitivity Cardiac Troponin T CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human High-sensitivity Cardiac Troponin T concentration in human serum.
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For in-vitro diagnostics purposes only

Background


The Human High-sensitivity Cardiac Troponin T CLIA Kit is designed, developed, and produced for the quantitative measurement of human High-sensitivity Cardiac Troponin T （hs-cTnT） level in serum samples. The assay utilizes a twosite “sandwich” technique with two antibodies that bind to different epitopes of hs-cTnT. Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated hs-cTnT polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immune-complex in the form of “magnetic particles– biotin hs-cTnT antibody–hs-cTnT–acridinium ester hs-cTnT antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a hs-cTnT in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum hs-cTnT concentration.

Specifications

Catalog no. SKT-050
Target Human High-Sensitivity Cardiac Troponin T CLIA Kit
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 8.00 pg/mL
Dynamic Range 8.0 pg/mL to 10000.0 pg/mL
Total Incubation Time 5 Minutes
Sample Type Serum
Sample Volume 50 µL
Storage Temperature 2-8 °C

Selected Literature


​​​​​​​1. Guo Huijia, Zhang Jianyi, Hu Yajun, et al. Correlation of high-sensitive cardiac troponin T with chronic heart failure and its differential significance for acute myocardial infarction[J]. Chin. Gen. Pract., 2014, 33(8):871-874.
2. Aviles RJ, Askari AT, LindahlB, et al. Troponin T levels in patients with acute coronary syndromes, with or without renal dysfunction. N Engl J Med, 2002, 346: 2047-205.
​​​​​​​3. Zhang Guofeng, Guo Yue, Guan Haixia, et al. Information other than laboratory results should be valued when diagnosing thyroid diseases:Lessons from the misdiagnosis of Graves' hyperthyroidism in a woman taking biotin. Chin. J. Endocrinol. Metab., 1991,68:1545–1550.

For in-vitro diagnostic use.