Human Anti-Thyroglobulin Antibody CLIA Kit

Background

The Human Anti-Thyroglobulin Antibody(Anti-Tg) CLIA Kit is designed, developed, and produced for the quantitative measurement of human Anti-Tg level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Anti-Tg.
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti-Tg polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles–biotin Tg antibody–Tg–acridinium ester Tg antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a Tg in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum Tg concentration.

Selected Literature

1.Mansourian AR. Metabolic pathways of tetraidothyronine and triidothyronine production by thyroid gland: a review of articles. Pak J Biol Sci, 2011, 14(1): 1-12.
2. Ruf J, Ferrand M, Durand-Gorde JM, et al. Significance of thyroglobulin antibodies cross-reactive with thyroperoxidase (TGPO antibodies) in individual patients and immunized mice. Clin Exp Immunol, 1993, 92(1): 65-72.

For in-vitro diagnostic use.