Human Ferritin CLIA Kit​​​​​​​

Description

​​​​​​​Human Ferritin CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human ferritin concentration in serum.

Background

The Human Ferritin CLIA Kit is designed, developed, and produced for the quantitative measurement of human Fer levels in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of Fer. Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the FER in the form of “magnetic particles–FER antibody–FER–acridinium ester FER antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a FER in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum FER concentration.

Selected Literature

​​​​​​​1.Goto F, Yoshihara T, Shjgemoto N, et al. Iron accumulation in rice seed by soya bean Fer gene. Nat Biotechnol, 1999, 17: 282-286.
2.Kong B, Huang HQ, Lin QM, et al. Electrophoretic Behavior, and Kinetics of Iron Release of Liver Fer of Dasyatisakajei. J Protein Chem, 2003, 22(1): 61 -71.
​​​​​​​ 3.llari A, Stefanini S, Chiancone E, et al. The dodecamericFer from Listeria innocua contains a novel intersubunit iron-binding site. Nat StructBiol, 2000, 7(1): 38 -43.

For in-vitro diagnostic use.