Human 25-Hydroxy Vitamin D CLIA Kit

Description

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Background

The Human 25-Hydroxy Vitamin D CLIA Kit is designed, developed, and produced for the quantitative measurement of human 25-OH VD level in serum samples. The assay utilizes competitive binding mechanisms for testing. The calibrator, control or sample, and acridine ester labeled 25-OH VD monoclonal antibody are mixed and incubated to form antigen-antibody immune complex. Streptavidin coated particles and biotinylated 25-OH VD were added under culture conditions. The unbound acridine ester labeled 25-OH VD monoclonal antibody of the sample binds to biotinylated 25-OH VD and binds to the microparticle through the biotin-streptavidin reaction. After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction. The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of 25-OH VD present in the sample. The test result is automatically calculated by the system according to the working curve.​​​​​​​

Selected Literature

​​​​​​​1. Zerwekh JE. Blood biomarkers of Vitamin D status. Am J ClinNutr, 2008, 87(suppl): 1087S-1091S.
2. Chapuy MC, Preziosi P, Maamer M, et al. Prevalence of Vitamin D insufficiency in an adult normal population. Osteoporosis Int, 1997, 7(5):439-443.
​​​​​​​3. Bischoff-Ferrari HA, Giovannucci E, Willett WC, et al. Estimation of optimal serum concentrations of 25-hydroxyvitamin D for multiple health outcomes. Am J ClinNutr, 2006, 84: 18-28.

For research use only. Not for use in diagnostics procedures.