SKT-034R

Human Neuron-specific enolase (NSE) CLIA Kit

Description

​​​​​​​
Human Neuron-specific enolase(NSE) CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Neuron-specific enolase concentration in serum.

For research use only. Not for use in diagnostics procedures.

Background


​​​​​​​The Human Neuron-specific enolase(NSE) CLIA Kit is designed, developed, and produced for the quantitative measurement of human NSE level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of NSE.
Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti-NSE polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immuno complex in the form of “magnetic particles–biotin NSE antibody–NSE–acridinium ester NSE antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then, trigger solutions are added to the reaction vessel and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a NSE in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum NSE concentration.

Specifications

Catalog no. SKT-034R
Target Human Neuron-specific Enolase
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD ≤0.050 ng/mL
Dynamic Range 0.050 ng/mL to 370.0 ng/mL
Total Incubation Time
Sample Type Serum
Sample Volume 20 µL
Storage Temperature 2-8 °C

Selected Literature


​​​​​​​1.PausE.,Nustadk.,Immunoradiometric,Assay,for,ay-Enolase (Neuron-Specific Enolase),with Use of Monoclonal Antibodies and Magnetizable Polymer Particles,Clin.Chem.,35,2034,1989.
2.Paus E., Risberg T., Establishment and Evaluation of a Radioimmunoassay for Neuron-Specific Enloase, Tumor Biol.10:23,1989.
3.Cooper E.H.,Pritchard J.,Bailey C.C.and Ninane J.Serum neuron-specific enolase in children'scancer. Br.J.Cancer56,65-67,1987.
For research use only. Not for use in diagnostics procedures.