SKT-022R

Progesterone CLIA Kit​​​​​​​

Description

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Progesterone CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human progesterone concentration in serum.
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​​​​​​​For research use only. Not for use in diagnostics procedures.

Background


The Progesterone CLIA Kit is designed, developed, and produced for the quantitative measurement of progesterone level in serum samples. The assay utilizes competitive bonding mechanisms for testing. The calibrators, controls, or sample, monoclonal antibody to Progesterone labeled with microparticles are mixed and incubated, forming an antigen-antibody immunocomplex.Acridinium ester labeled Progesterone are added and incubated.Acridinium ester labeled Progesterone analog unbound to the sample binds to the Magnetically microgranular Progesterone monoclonal antibody,After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction. The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of progesterone present in the sample. The test result is automatically calculated by the system according to the working curve.

Specifications

Catalog no. SKT-022R
Target Progesterone
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD ≤0.100 ng/mL
Dynamic Range 0.100 ng/mL to 40.00 ng/mL
Total Incubation Time 20 Minutes
Sample Type Serum
Sample Volume 50 µL
Storage Temperature 2-8 °C

Selected Literature


​​​​​​​1.Meyers FH, Jawetz E, Goldfien A. Review of Medical Pharmacology, 6th Edition, 1978, 38: 402.
2. Wintrobe MM. Principles of Internal Medicine, 7th Edition, 1974, 92: 577.
​​​​​​​3. Frtiz F, Klopper A. Endocrinology of Pregnancy, 2nd Edition, 1977, 6: 99-122.
For research use only. Not for use in diagnostics procedures.