Human chorionic gonadotropin and β subunit CLIA Kit

Description

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Background

The human chorionic gonadotropin and β subunit CLIA Kit is designed, developed, and produced for the quantitative measurement of HCG+β level in serum samples. The assay utilizes a two-site “sandwich” technique with two antibodies that bind to different epitopes of HCG+β.
​​​​​​​Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the HCG+β in the form of “magnetic particles–HCG+β antibody–HCG+β– acridinium ester HCG+β antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a HCG+β in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum HCG+β concentration.

Selected Literature

​​​​​​​1.Russell PT. Pregnancy and fetal function. In: Kaplan LA, Pesce AJ, editors. Clinical chemistry: theory, analysis, and correlation. 2nd ed. St. Louis: CV Mosby, 1989, 572.
2. Kaplan LA. Human chorionic gonadotropin. In: Kaplan LA, Pesce AJ, editors. Clinical chemistry: theory, analysis, and correlation. 2nd ed. St. Louis: CV Mosby, 1989, 938–944.
​​​​​​​3.Sturgeon CM, McAllister EJ. Analysis of hCG: clinical applications and assay requirements. Ann Clin Biochem, 1998, 35: 460-491.

For research use only. Not for use in diagnostics procedures.