Human Estradiol CLIA Kit​​​​​​​

Description

​​​​​​​Human Estradiol CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitativemeasurement of human estradiol concentration in serum.

Background

The Human Estradiol CLIA Kit is designed, developed, and produced for the quantitative measurement of human E2 level in serum samples. The assay utilizes the competitive binding mechanism for testing.
The calibrators, controls, or sample, monoclonal antibody to E2 labeled with microparticles are mixed and incubated, forming an antigen-antibody immuno-complex. Acridinium ester labeled E2 analog unbound to the sample binds to the Magnetically microgranular E2 monoclonal antibody , After precipitation in a magnetic field, the supernatant is decanted, and then a wash cycle is performed to remove any remaining substances that are not bound to the magnetic microbeads. Subsequently, the washed compound is sent into the measurement chamber where trigger solution is automatically added to initiate a chemiluminescence reaction.
The light signal is measured by a photomultiplier as relative light units (RLUs), which is inversely proportional to the concentration of E2 present in the sample. The test result is automatically calculated by the system according to the working curve.
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Selected Literature

​​​​​​​1. Sperof FL, Glass RH, Kase NG, editors. Clinical gynecologic endocrinology and infer tility. 4th ed. Baltimore: Williams and Wilkins, 1989, 91–119.
2. Mohr IJ, Clark R, Sun S, et al. Targeting the E1 replication protein to the papillomavirus origin of replication by complex formation with the E2 transactivator. Science, 1990, 250(4988): 1694-1699.
​​​​​​​3. Lorick KL, Jensen JP, Fang S, et al. RING fingers mediate ubiquitinconjugating enzyme (E2)-dependent ubiquitination. ProcNatlAcadSci U S A., 1999, 96(20): 11364-11369.

For in-vitro diagnostic use.