Human Luteinizing Hormone CLIA Kit

Background

The Human Luteinizing Hormone CLIA Kit is designed, developed, and produced for the quantitative measurement of human LH level in serum samples. The assay utilizes a twosite “sandwich” technique with two antibodies that bind to different epitopes of LH.
​​​​​​​Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with magnetic particles antibody. The magnetic particles capture the LH in the form of “magnetic particles–LH antibody–LH–acridinium ester LH antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 or ECL 25 analyzer. The relative light units (RLU) are proportional to the concentration of a PRL in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum LH concentration.

Selected Literature

​​​​​​​1. Johnson MR, Carter G , Grint C .Relationship between ovarian steroids gonadotropin and rela ting during the menstrual cycle .ActaEndocrinol , 1993, 129(2):121-125.
2. Marina LV, Ivović M, Tančić-GajićM.Luteinizing hormone and insulin resistance in menopausal patients with adrenal incidentalomas : The cause-effect relationship ClinEndocrinol (Oxf ), 2018, 88 (4): 541-548.
​​​​​​​3. Casarini L, Santi D, SimoniM.'Spare' l uteinizing h ormone r eceptors: f acts and f iction.TrendsEndocrinolMetab , 2018, 29 (4): 208-217.

For research use only. Not for use in diagnostics procedures.