SKT-015

Human Thyroid Stimulating Hormone CLIA Kit​​​​​​

Description

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Human Thyroid Stimulating Hormone CLIA Kit is a Chemiluminescence Immunoassay (CLIA) intended for the quantitative measurement of human Thyroid Stimulating Hormone in serum.
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For in-vitro diagnostics purposes only

Background


​​​​​​​​​​​​​​The Human Thyroid Stimulating Hormone CLIA Kit is designed, developed, and produced for the quantitative measurement of human TSH level in serum samples. The TSH assay is a two-site sandwich assay to determination the level of follicle-stimulating hormone. Assay calibrators, controls, or patient serum samples are added directly to a reaction vessel together with streptavidin coated magnetic particles and biotinylated anti- TSH polyclonal antibody. The magnetic particles capture the biotin antibody as well as an immune-complex in the form of “magnetic particles– biotin TSH antibody–TSH–acridinium ester TSH antibody”. Materials bound to the solid beads are held in a magnetic field while unbound materials are washed away. Then trigger solutions are added to the reaction vessel, and light emission is measured with the ECL100 analyzer. The relative light units (RLU) are proportional to the concentration of a TSH in the sample. The amount of analyte in the sample is determined from a stored, multi-point calibration curve and reported in serum TSH concentration.


Specifications

Catalog no. SKT-015
Target Thyroid
Species Human
Method Sandwich CLIA
Tests Per Kit 100 tests
Detection Flash AE Chemiluminescence
Sensitivity / LLOD 0.05μIU/mL
Dynamic Range 0.05μIU/mL to 100.0μIU/mL
Total Incubation Time 30 minutes
Sample Type Serum
Sample Volume 50 µL
Storage Temperature 2-8 °C

Selected Literature


​​​​​​​​​​​​​​1. Watts NB, Keffer JH. Practical endocrine diagnosis. 3rd ed. Philadelphia: Lea and Febiger, 1982, 77–96.
2. Fernandez-Ulloa M, Maxon HR. Thyroid. In: Kaplan LA, Pesce AJ, editors. Clinical chemistry: theory, analysis, and correlation. 2nd ed. St. Louis: CV Mosby, 1989, 620–637.
​​​​​​​3. White GH. Recent advances in routine thyroid function testing. Crit Rev Cl Lab Sci, 1986, 24(4): 315-362.
For in-vitro diagnostic use.